BACE - Leaf Litter

Jen contacted Jeff Dukes at Purdue to get leaf litter from their grassland site in Boston.

Hopefully, this will be shipped next week (11/17).
***Claudia sent them a box on 11/13/14***

Need to find the leaf litter media from lab intranet (Kristen could help).

Found the following:

-       Grind litter for 30 seconds to break into smaller chunks. Continue until you have ~200ml of ground litter.

-       Add litter and 1 liter of DI water to large flask. Cover top with foil.

-       Place on stir plate for 24 hours.

-       Allow litter to settle for 24-48 hours.

-       Decant liquid (siphon or scoop) into clean flask (you should have ~800 ml of media).

-       Filter media through 100 μm, 8 μm, 3 μm, and 0.8 μm membranes (I use larger sizes first to decrease the use of expensive 0.8 μm filters).

-       Transfer media into autoclavable jug. Add 15g agar and fill with DI water until final volume reached 1 litter.

-       Autoclave (Liquid cycle)