Curto - PCR

Took Kristen's Curtobacterium strains from deep freeze and plated.

Performed PCR:

	1 Rxn (μl)	Rxn Number	Total
		12
dH2O	13.3		159.6
Premix F	15		180
pA (50μM)	0.2		2.4
pH' (50μM)	0.2		2.4
Taq (5units/μl)	0.3		3.6
DNA	1

rDNA 16S Gene for 1500 bp 

Primers Used:

Forward Primer - pA -> 27f / E8F

Reverse Primer - pH' -> 1525f / E1541F

5 of the 9 strains amplified

Need to reevaluate strains that failed