BACE - Culture litter

1. Took Boston litter and ran through autoclaved sieves (2000 um -> 250 um -> 25 um)
1. ***NEXT TIME - GRIND UP LITTER BEFOREHAND
2. Scraped water and residue in bottom two filters into 50mL conicals
3. Add litter residue to vacuum filter (no vacuum attached) with 100 um filter
4. Rinse conicals 2x with autoclaved DI H2O
5. Total of 200 mL of litter residue and add to new set of 50mL conicals
6. Dilute each tube to 1x, 1:100, 1:1000, 1:10000
7. Plate 100 uL of each dilution on media plates:
1. For this experiment, plated on both Loma Ridge Media and BACE Media